DR. Antoine Sayegh new experiments in cancer:

DR. Antoine Sayegh new experiments in cancer:
1.the hybridization phase :
We incubate together in the Petri dishes which nourished with high amounts of Argenin by promulgation the rules of the Lederberg-Tatum experiment and Bernard Davis U tube experiment
a. the aerobic types strains of the denitrifies bacteria like pseudomonas stutzeri TR2 or the strains paracocus denitryficans K50 to produce the nitrous oxide and in the tissues the formation of the NO came from the converting L-Argenine by the enzyme nitric oxide synthase But the NO in the tissues plays an important roles in the cancers because the macrophages :these cells activated by the cytokines or by the TNF which produces the nitric oxide ( NO ) which kills the organisms ( for the aids viruses also) and the malignant cells ( cancers )and the macrophages which aid and which did not aid also by the antibodies.
Or from the uses:
1. the ammonia oxidizing bacteria( AOB)which produce the nitrates from the Ammonium
2.the sulfur oxidizing bacteria as Thiobacillus denitrificans which express both form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO).
b. the bacteria( different cyanide types of the pseudomonas ) which have the abilities to digest the cyanide by the enzyme pterin – dependent hydroxylase which breaks the nitrogen out of the cyanide and these bacteria can form the cyanide from the Glycin.
c. different types of the mediators which facilitate the hybridization like :
a. RNase III proteins which act as endonuclease.
b. ERA proteins to facilitate the growth and the divisions by its GTPase domain and the RNA –binding KH domain .
c. magnesium with the different types of the endonuclease and DNA ligase also or we can use also the HPPK type of the pyrophosphokinase enzyme which acts for the folate biosynthetic pathway which needed for the microorganisms or to use the Nus family which interacts with protein – protein or protein –RNA by its types the A,B,E,G especially by the formation complex NusB-NusE-dsRNA in the assemblies complex as antitermiantion complex .
This phase help us to create a new strains of the bacteria( the hybrid bacteria) which have the ability to depend on the cyanide in its nourishments and can form the Nitric oxide( NO) in the same time to use them later .
d.( very important step) to confirm the formation of the high concentration of NO in the hybrid bacteria that we can use the activated different types of the reductase enzymes especially the NIR types which convert the NO2 to NO or by suppress the other reductase enzymes with the specific anti bodies to increase the formation only the NO in the hybrid bacteria ,the reductase enzymes came from the genes NIRs ,NORcb , NORz, NARx, NARL Because these Specific enzymes (Nar, Nir, Nor, Nos) play an important roles in the process of denitrification. Redox reactions Nar- nitrate to nitrite,Nir- nitrite to nitrogen monoxide,Nor- nitrogen monoxide to nitrous oxide, Nir- nitrous oxide to atmospheric nitrogen .

2. the cellular phase :
a. in vitro :
we incubate in the same time in a special dishes ( Petri dishes without Agar but with the serum of the blood of the same patient which has the cancer tumor) many cancer cells and many normal cells from the same tumor with the hybrid bacteria with the same conditions of the Lederberg-Tatum experiment and Bernard Davis U tube experiment and we add also the p53 proteins, the cells the macrophages and the natural killer cells ( NK ) and the killer cells ( K ) and the LAK cells by prepare them from many steps:
1 step 1: we use colony stimulating factors to increase the number of the W.B.C. to use many types later. we isolate the cells the macrophages and the natural killer cells ( NK ) and the killer cells ( K ) to prepare them for the research.
2.step 2 :we use the chemo tactic factors :
a. the n-formyl methionin contains peptides as chemo tactic powerful action for phagocytes.
b. the intergrins that help for promote the binding the phagocytes to the endothelial cells in the tumors
c. the humors factors like the interferon ,TNF, IL2, C5a to help for the increase the phagocyte actions.
3.step 3 : we prepare the cancer tissues (small amounts or few cells ) with these factors and we put with them the types of the killer cells before to increase and excite its functions and to get the LAK cells
the properties of the cells
1. the macrophages :these cells activated by the cytokines and it produce the TNF which produce the nitric oxide ( NO ) that kill the organisms and the malignant cells these cells also does not aid by the antibodies .
2. the natural killer cells : NK : which has low affinity for the fc receptors and its types the CD56 ,CD16 it kill the aids viruses and the cancer cells by its cytolytic proteins the ( perforin ) and it contains large granules and it called the lymphokine activated killer cells the ( LAK ) that mean its origin from the CD56,CD16 and by activation by the interferon and IL2 .
3.the killer cells :K :these cells depend on the fc receptors and kill the malignant cells coated by the IGG to cause the lyses

b. in vivo :
1. we inject the hybrid bacteria ( as vaccine) subcutaneously to increase the immunity of the patients by formation the IGM and IGG later to avoid any harms of the hybrid bacteria .
2. we inject the hybrid bacteria again into the blood of the patient directly after many days.
3. we inject also with the hybrid bacteria with many of the normal cells of the same tissue of the cancer ( from biopsy ) the p53 proteins, the cells the macrophages and the natural killer cells ( NK ) and the killer cells ( K ) and the LAK cells .
4. we can use the PABA/NO which activated by the glutathione S- tranferase ( GST) by its types the GST-alpha and GST-pi to kill cancer cells by releasing cytotoxic levels of nitric oxide .
5. to confirm the real death of the cancer cells we can use ( both in vivo and in vitro) the TNF which secreted from the macrophages and the monocytes can activate the TNF-R1 receptor and induce the apoptosis ( the immune complexes which mediates the IL1 and the IL2)
Abstract on The preparing of the cancer cells for apoptosis:
1.the activation of the MAPK pathway by the cascades activations of the MEK proteins and the ERK1,2 proteins which activates the genes
a. MYC,MYB for the cellular growth .
b. FOS ,JUN, for induces the apoptosis .
2. the over activities of the protein TP53 which activates the proteins BAX,NOXA,PUMA which activates the apoptosis.
3.the mutations in the RB1 genes or loss of it help for over activation for the E2F1 which activate the ways for the apoptosis .
4.the RAS protein which acts as a switch between many oncogenes pathways which activates the NORE1, RASSIA which leads for the apoptosis.
5.The anti apoptosis proteins :
a. BCL2( its type BCL-W) inactivates the TP53.
b. BCL-XL (its type MCL-1,A1,NRF3) activates the NFkB.
6. the pro-apoptotic proteins :
a. BAX protein (its type BAK)activate TP53,and (its type BOK ) inactivates the AKT protein .
b. the BID protein activates the caspasis 8, and the PUMA activates the TP53 ,and the NIX protein regulates the hypoxia
7.the mechanisms which disrupt the apoptosis in the cancer cells play an important roles for these cells survivals :
a. mutations in the death receptors in the extrinsic pathway.
b. mutations or disruptions in the genes which form the proteins for the intrinsic pathway .
c loss or defect in the genes TP53.
d. over expression of the protein IAPs.
Activation for the anti-apoptotic pathways .
From these important notes we can prepare the cancer cells for the apoptosis by the next steps:
1. we take normal tissues of the same type of the cancer cells and we excite in it the normal pathways for the cellular death by:
a. excite the death receptors for the normal tissues ( out of the body) by its specific growth factors.
b. by formation the immune complexes ( antigens with the anti bodies with the complements ( different types ) as in the researches before to destroy these normal cells by the apoptosis pathways .
2. we isolate the excited proteins from the residues of the death cells ,because these proteins contain multi types of the apoptotic proteins with low types of the anti-apoptotic proteins ,to use it later as( switch proteins ) .
3. in the cancer cells with the inappropriate proliferations and differentiations which excite the genes of the anti-apoptotic proteins like the BCL2 families , so we find high concentrations of these proteins in the cancer cells( due for many divisions) while we find it with low concentrations in the normal cells .
4.the most important notes in the process of the apoptosis:
a. the important roles of the P53 protein in the inducing the BAX ,NOXA,PUMA in the mitochondria and the BAX is the antagonist for the BCL2 in the intrinsic pathway ,so the P53 protein help us for the apoptosis .
b. the importance of the death receptors FAS/CD95 , DR4/DR5, DR3, TNF and its adaptors the FAAD and the TRADD and the activators which bind the of the death ligands like the FASL/CD95 , TRAIL/APO-2L ,APO-3L , TF ,so the uses especially of the TRAIL ( which acts on the death receptors the TRAIL-R1 and the TRAIL-R2 ) proteins with the high toxicities on the normal cells especially the hepatocytes ,so the uses of the TNF which secreted from the macrophages and the monocytes can activate the TNF-R1 receptor and induce the apoptosis ( the immune complexes which mediates the IL1 and the IL2).
c .as the RAS play an important actions for the connections between many oncogenes pathways and activates the NORE1 and the most important RASSF1A protein (which form from the 3p21,3 chromosome and binds to the microtubules which block the mitosis ,and the activation of the apoptosis by reverse the actions of the AKT proteins which prevents the BAD protein to attach the mitochondrial proteins and prevents also the FKHR-L1( which activates the apoptosis) to enters the nucleus ,so the uses of the P53 , the RASSF1A, FKHR-L1 proteins in our researches and attack the AKT proteins with the antibodies ( or the isotopes ) can we change the directions from the activations of the cellular divisions from the oncogenes pathways for activations the different mechanisms of the apoptosis in the same times ( the synergistic effects )
5.we use the researches before like :
a. The complete weakness for the cancer cells in the part II.
b. The new treatments for aids and cancer and genes deformities by the mutated promoters in the part II.
c. The theoretic research on the cellular immunity in the part I.
5. after exciting for the killer cells and make the weakness in the cancer cells we direct these cells for the apoptosis by using the switch proteins because it contains high doses of the apoptotic proteins ,to reverse and neutralize the anti-apoptotic proteins ( part of the switch proteins ) and the ratio between the apoptotic and anti –apoptotic proteins directs the cancer cells for the apoptosis ,and because the reverse actions of the anti-apoptotic proteins by the switch proteins with the high concentrations ,helping for activations also the E2F1,and the oncogenes pathways for activations the FOS ,JUN genes to complete the apoptosis ,and to facilitate the actions of the killer cells .
The important notes :
1. the immune complex needs the antigens ,antibodies ,and the complement system ,but in many times it needs the super antigen to evoke the immune response, due to complete the proteins balances ( electromagnetic balances ,due to the different charges on the protein surfaces , attractive or incongruity )by filling the empty gaps in the immune complexes
2. DAXX pathways :the excitation of the apoptosis from TGFb by the phosphorylation of the DAXX protein ,with the hipk2 and the ask1 to activates the MAP2K3 ,with p53 ,ands to the apoptosis( the pathway 1) ,while in the another pathway ( the pathway 2 ) which did not depend on the P53 only from the MAPK8 ,ends to the apoptosis .
From these two examples , we find in the proteins balances ,different types of the proteins needed to continue the cascades ,it means , the array of the proteins depends on the different types with the different charges ,so the balances in the protein cascades very important to achieve the targets , so we can get an important benefits in our researches .
3. in the preparing the cancer cells for the apoptosis ,we uses the switch proteins to oblige the cancer cells for the apoptosis , with the uses of the different types of the proteins as before ,these deferent types of the proteins has different types of the residues( the P53 has rich prolin in its domain ,may be suitable for many residues and not suitable for the others ), and in its end sides different types of the charges, so the attractive and incongruity forces plays an important roles in the array of the proteins in the cascades of the apoptosis , and to solve this problems we must do:
a. we uses small series of the amino acids or very short proteins with different amino acids in its ends to fill the gaps between the proteins cascades and to modify the charges , to oblige the cancer cell for the apoptosis ,by cutting the long series of the proteins by the proteases enzymes or different types of the kallekrien.
b. by the uses of the multi artificial of the short series of the nucleic acid of the messenger RNA to form these short proteins with normal ways .
c. we can use the amino acids with isotopes to isolate and recognize the active types of the short proteins ( the structure sand the residues) .
d .we use these types of the super proteins ( the links proteins ) with great amounts and with different randomly lengths, to get more benefits in the filling the electromagnetic gaps between the proteins in the cascades to discover the active types
4.one of the most important steps, for the successful for the apoptosis, the uses of the proteins :
a. the Cro proteins which formed from the viruses for the lytic cells for the host cells ,so we can use these types of the proteins beside the other steps before, for the cancer cells.
b. we can use the proteins which formed from the retro viruses ,the Tat types which activates the formation or the activation the TRAIL which mediates the apoptosis .
so we can activate the cancer cells for the apoptosis and the damages by these proteins which participate for the apoptosis by the normal ways without the uses of the vital organisms .

The aims of the experiments :
To kill the cancer cells in the malignant tissues from the hybrid bacteria by the intrinsic cyanide and from the nitric oxide (NO) and to clean the body from the residues of the destroyed cells of cancer by normal mechanisms of human immune system from the large affinity of the hybrid bacteria due to the excess vessels in the cancer tissues and from the specific qualities of the NO on the vascular system also.
With the best regards from:
Ambassador .H . E. Dr .Antoine Sayegh MD
Syria Blue Crescent Society
Director / Delegate FHM Medical Attaché.
Faculty of Humanitarian Medics (FHM)
Director for Syrian Human Rights Council(SHRC)
The strongest international defender of the world for human rights
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